Oxidative radioiodination damage to human lactoferrin

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Oxidative radioiodination damage to human lactoferrin.

Oxidative iodination of human lactoferrin (Lf) as commonly performed by using the chloramine-T, the Iodogen or the lactoperoxidase method produces an unreliable tracer protein because of excessive and heterogeneous polymer formation. Before iodination a minor tetramer fraction may be demonstrable in iron-saturated Lf only. Iodination-induced polymerization of iron-poor as well as iron-saturated...

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Oxidative DNA damage in human sperm influences time to pregnancy.

BACKGROUND Oxidative stress and related DNA damage in human sperm may be important for fecundity and pregnancy outcome. METHODS We studied the level of oxidative DNA damage in terms of 7-hydro-8-oxo-2'-deoxyguanosine (8-oxodG) in sperm DNA among 225 first-pregnancy planners. Over the six menstrual cycle follow-up time, after cessation of contraception, 135 pregnancies were conceived. RESULT...

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Oxidative damage in human gingival fibroblasts exposed to cigarette smoke.

Cigarette smoke, a complex mixture of over 7000 chemicals, contains many components capable of eliciting oxidative stress, which may induce smoking-related disorders, including oral cavity diseases. In this study, we investigated the effects of whole (mainstream) cigarette smoke on human gingival fibroblasts (HGFs). Cells were exposed to various puffs (0.5-12) of whole cigarette smoke and oxida...

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Bovine lactoferrin ameliorates ferric nitrilotriacetate-induced renal oxidative damage in rats

Milk provides a well-balanced source of amino acids and other ingredients. One of the functional ingredients in milk is lactoferrin (LF). LF presents a wide variety of bioactivities and functions as a radical scavenger in models using iron-ascorbate complexes and asbestos. Human clinical trials of oral LF administration for the prevention of colon polyps have been successful and demonstrated th...

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Biomarkers of oxidative damage in human disease.

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1986

ISSN: 0264-6021,1470-8728

DOI: 10.1042/bj2400239